Conference Portal, 2018 International Conference on Pure and Applied Science

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Expression patterns of GUS Gene in Five Different Strains of Arabidopsis thaliana Plants.
Hikmat M. Masyab

Last modified: 2018-08-16

Abstract


In response to the threats caused by phytopathogens and plant disease, this paper aims to look at the potential of genetic modification technology as a strategy to protect plant crops globally. Specifically, it will explore the usefulness of GUS (β-Glucuronidase) as reporter genes in GUS staining of 5 separate strains of Arabidopsis thaliana which used to identify promoters present and their site of action. GUS expression patterns had been studied in 5 different strains of Arabidopsis thaliana plants to determine which promoter the plant is carrying. Using a GUS stain, the genetics behind each unknown plant can experimentally be deduced. In this paper, 5 previously generated strains, each with different promoters (Col 0, ATAO1, ARR6:GUS, 35S:GUS, GL2:GUS) and integration location of the transgene, will show different expression patterns.

 

Published 01 August 2018

DOI: 10.14500/icpas2018.bph24

http://dx.doi.org/10.14500/icpas2018.bph24


Keywords


Arabidopsis thaliana; Biotechnology; Gene expression; β-Glucuronidase

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