Conference Portal, 2018 International Conference on Pure and Applied Science

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Isolation of plasmid DNA from Antibiotics Resistant Staphylococcus aureus
Hanan T. Subhi, Fatimah R. Abdel, Ihsan A. Raheem

Last modified: 2018-08-14


Staphylococcus aureus is an opportunistic organism responsible for most nosocomial infection. From a total of 100 staphylococci isolates, 41 were S. aureus collected from 8 (19.5%) tonsillitis, 9 (22%) Skin , 13 (31.7%)  wound, 6 (14.6%)  nose and 5 (12.2%) from ear infection samples. All bacterial isolates were identified by the biochemical, cultural and microbial characteristics confirmed by Api Staph System. β-lactamase test of S. aureus revealed that (21) isolates were positive. While 31 (75.61%) were able to produce urease. The sensitivity test of four quinolones groups showed that (50.2%) isolates were resistant to norfloxacin, (44.1%) were resistant to ofloxacin and (39.8%) were resistant to ciprofloxacin. While the lowest resistance was (25.7%) to levofloxacin.  The β-lactamase positive S. aureus showed a high resistance in compare to that β-lactamase negative isolates. The range of the minimum inhibitory concentrations of levofloxacin was (16-512 µg\ml). A single plasmid  was detected in tow isolates with same size. The DNA plasmid was determined from levofloxacin resistant isolates.


Published 01 August 2018

DOI: 10.14500/icpas2018.mim105


Antibiotic resistance; Levofloxacin; Plasmid; Staphylococcus aureus; β-lactamase

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