Last modified: 2018-08-16
Abstract
In response to the threats caused by phytopathogens and plant disease, this paper aims to look at the potential of genetic modification technology as a strategy to protect plant crops globally. Specifically, it will explore the usefulness of GUS (β-Glucuronidase) as reporter genes in GUS staining of 5 separate strains of Arabidopsis thaliana which used to identify promoters present and their site of action. GUS expression patterns had been studied in 5 different strains of Arabidopsis thaliana plants to determine which promoter the plant is carrying. Using a GUS stain, the genetics behind each unknown plant can experimentally be deduced. In this paper, 5 previously generated strains, each with different promoters (Col 0, ATAO1, ARR6:GUS, 35S:GUS, GL2:GUS) and integration location of the transgene, will show different expression patterns.
Published 01 August 2018